Abstract
An immunodiffusion test was developed for diagnosing subcutaneous and systemic pythiosis in humans. When culture filtrate antigen (CFA) from P. insidiosum was reacted against patient and rabbit antisera, 1–5 precipitin bands occured both in patient and rabbit antisera, and a line of identity also occured between patient and rabbit sera. When control P. insidiosum CFA was reacted with 30 apparently normal persons, 20 Thalassemia patients, 2 candidosis and 5 aspergillosis patients, no precipitin bands were found. P. insidiosum CFA also tested with rabbit antibodies to B. dermatitidis, C. immitis, H. capsulatum, P. brasiliensis, C. albicans, M. furfur and A. fumigatus revealed no cross reactions. This test is practical, sensitive and specific.
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References
Mendoza L, Alfaro AA. Equine pythiosis in Costa Rica: Report of 39 cases. Mycopathologia 1986; 94: 123–9.
Smith F. The pathology of bursattee. Vet J 1884; 19: 16–17.
Haan J, Hoogkamer LJ. Hyphomycosis destruens equine. Archiv Wiss Prakt Tierheilk 1903; 29: 395–410.
Bridges CH, Emmons CW. A phycomycosis of horse caused by Hyphomyces destruens. J Am Vet Med Assoc 1968; 138: 579–89.
Hutchins DR, Johnston KG. Phycomycosis in the horse. Aust Vet J 1972; 48: 269–78.
Austwick PKC, Copland JW. Swamp cancer. Nature 1974; 250: 84.
Chandler FW, Kaplan W, Ajello L. A color atlas and textbook of the histopathology of mycotic diseases. Chicago: Year Book Medical Publishers Inc., 1980; 104–5.
Mendoza L, Kaufman L, Standard PG. Immunodiffusion test for Diagnosis and Monitoring Pythiosis in Horses. J Clin Microbiol 1986; 23: 813–6.
Thianprasit M. Cutaneous Pythiosis in Human-Being. Abstract in Siriraj 100th Academic Meeting, Mahidol University, Bangkok, Thailand, 18–22 April 1988: 94.
De Cock AWAM, Mendoza L, Padhye AA, Ajello L, Kaufman L. Pythium insidiosum sp. nov., the etiologic agent of pythiosis. J Clin Microbiol 1987; 25: 344–9.
Sathapatayawongs B. Human Pythiosis. Siriraj 100th Academic Meeting, Mahidol University, Bangkok, Thailand, 18–22 April 1988: 258–62.
Miller RI, Campbell RSF. Immunological studies on equine phycomycosis. Aust Vet J 1982; 58: 227–31.
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Imwidthaya, P., Srimuang, S. Immunodiffusion test for diagnosing human pythiosis. Mycopathologia 106, 109–112 (1989). https://doi.org/10.1007/BF00437089
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DOI: https://doi.org/10.1007/BF00437089