Skip to main content
SpringerLink
Log in

Proteins of the extracellular matrix in vitreoretinal membranes

  • Laboratory Investigations
  • Published:
Graefe's Archive for Clinical and Experimental Ophthalmology Aims and scope Submit manuscript

Abstract

Epiretinal and vitreous membranes of different etiology, e.g., in diabetic retinopathy, following retinal detachment, trauma or inflammatory processes, show a similar morphology. The exact composition of the extracellular matrix and the pathogenesis of these membranes remain uncertain. The presence of collagens, type I-IV, laminin, and fibronectin can be shown by means of immunofluorescence with affinity-purified antibodies. Collagen type V was revealed by SDS-polyacrylamide-gel electrophoresis. These proteins of the extracellular matrix have diverse properties and functions in the membranes, as is discussed. Despite great similarities in morphology, there are some differences in the matrix, seemingly dependent upon the etiology of the membrane.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bentz H, Bächinger HP, Glanville R, Kühn K (1978) Physical evidence for the assembly of A and B chains of human placental collagen in a single triple helix. Eur J Biochem 92:563–567

    Google Scholar 

  2. Campochiaro PA, Jerdan JA, Glaser BM (1984) Serum contains chemoattractants for human retinal pigment epithelial cells. Arch Ophthalmol 102:1830–1833

    Google Scholar 

  3. Campochiaro PA, Jerdan JA, Glaser BM (1986) The extracellular matrix of human retinal pigment epithelial cells in vivo and its synthesis in vitro. Invest Ophthalmol Vis Sci 27: 1615–1621

    Google Scholar 

  4. D'Ardenne AJ, McGee JOD (1984) Fibronectin in disease. J Pathol 142: 235–251

    Google Scholar 

  5. Furthmayr H, Mark K von der (1982) The use of antibodies to connective tissue proteins in studies on their localization in tissues. In: Furthmayr H (ed) Immunochemistry of the extracellular matrix, vol II. CRC Press, Boca Raton, Fla, pp 95–108

    Google Scholar 

  6. Green WR, Kenyon KR, Michels RG, Gilbert HD, Cruz Z de la (1979) Ultrastructure of epiretinal membranes causing macular pucker after retinal reattachment surgery. Trans Ophthalmol Soc UK 99:63–77

    Google Scholar 

  7. Grinnell F, Billingham RE, Burgess L (1981) Distribution of fibronectin during wound healing in vivo. J Invest Dermatol 76:181–189

    Google Scholar 

  8. Hiscott PS, Grierson I, McLeod D (1985) Natural history of fibrocellular epiretinal membranes: a quantitative, autoradiographic, and immunohistochemical study. Br J Ophthalmol 69: 810–823

    Google Scholar 

  9. Jerdan JA, Michels RG, Glaser BM (1986) Diabetic preretinal membranes. An immunohistochemical study. Arch Ophthalmol 104:286–290

    Google Scholar 

  10. Kampik A (1982) Klinische und pathologische Aspekte epiretinaler Membranbildungen. Klin Monatsbl Augenheilkd 181:386–387

    Google Scholar 

  11. Kampik A, Green WR, Michels RG, Nase PK (1980) Ultrastructural features of progressive idiopathic epiretinal membrane removed by vitreous surgery. Am J Ophthalmol 90: 797–809

    Google Scholar 

  12. Kampik A, Kenyon KR, Michels RG, Green WR, Cruz ZC de la (1981) Epiretinal and vitreous membranes. Comparative study of 56 cases. Arch Ophthalmol 99: 1445–1454

    Google Scholar 

  13. Kenyon KR, Michels RG (1977) Ultrastructure of epiretinal membrane removed by pars plana vitreoretinal surgery. Am J Ophthalmol 83:815–823

    Google Scholar 

  14. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Google Scholar 

  15. Laqua H, Machemer R (1975) Glial cell proliferation in retinal detachment (massive periretinal prolieration). Am J Ophthalmol 80:602–618

    Google Scholar 

  16. Li W, Stramm LE, Aguirre GD, Rockey JH (1984) Extracellular matrix production by cat retinal pigment epithelium in vitro: characterization of type IV collagen synthesis. Exp Eye Res 38:291–304

    Google Scholar 

  17. Machemer R, Laqua H (1975) Pigment epithelium proliferation in retinal detachment (massive periretinal proliferation). Am J Ophthalmol 80:1–23

    Google Scholar 

  18. Mark K von der (1981) Localization of collagen types in tissues. In: Hall DA, Jackson DS (eds) International Review of Connective Tissue Research, vol 9. Academic Press, New York London Paris, pp 265–324

    Google Scholar 

  19. Mark H von der, Mark K von der, Gay S (1976) Study of differential collagen synthesis during development of the chick embryo by immunofluorescence. I. Preparation of collagen type I and collagen type II specific antibodies and their application to early stages of the chick embryo. Dev Biol 48: 237–249

    Google Scholar 

  20. Mark K von der, Meuxel M van, Wiedemann H (1982) Isolation and characterization of new collagens from chick cartilage. Eur J Biochem 24:57–62

    Google Scholar 

  21. Marshall T (1984) Detection of protein in polyacrylamide gels using an improved silver stain. Ann Biochem 136:340–346

    Google Scholar 

  22. Modesti A, Kalebic T, Scarpa S, Togo S, Grotendorst G, Liotta LA, Triche TJ (1984) Type V collagen in human amnion is a 12 nm fibrillar component of the pericellular interstitium. Eur J Cell Biol 35:246–255

    Google Scholar 

  23. Newsome DA, Linsenmayer TF, Trelstad RL (1976) Vitreous body collagen. Evidence for a dual origin from the neural retina and hyalocytes. J Cell Biol 71:59–67

    Google Scholar 

  24. Norton EWD (1969) Complications of retinal detachment surgery. In: Symposium on retina and retinal surgery. Transactions of the New Orleans Academy of Ophthalmology. Mosby, St Louis, pp 222–234

    Google Scholar 

  25. Nowack H, Gay S, Wick G, Becker U, Timpl R (1976) Preparation and use in immunohistology of antibodies specific for type I and type III collagens. J Immunol Methods 12:117–124

    Google Scholar 

  26. Rennard SI, Berg R, Martin GR, Foidart JM, Robey PG (1980) Enzyme-linked immunoassay (ELISA) for connective tissue components. Anal Biochem 104:205–214

    Google Scholar 

  27. Ruoslahti E, Engvall E, Hayman EG (1981) Fibronectin: current concepts of its structure and function. Coll Relat Res 1:95–128

    Google Scholar 

  28. Scheiffarth OF, Günther H, Kampik A, Mark K von der, Timpl R (1986) Strukturproteine bei proliferativer Vitreoretinopathie. Fortschr Ophthalmol 83:582–584

    Google Scholar 

  29. Smith RS, Heuwen WAJ van, Streeten B (1976) Vitreous membranes: a light and electron microscopical study. Arch Ophthalmol 94:1556–1560

    Google Scholar 

  30. Terranova VP, Rohrbach DH, Martin GR (1980) Role of laminin in the attachment of PAM 212 (epithelial) cells to basement membrane collagen. Cell 22:719–726

    Google Scholar 

  31. Timpl R, Wick G, Gay S (1977) Antibodies to distinct types of collagens and procollagens and their application in immunohistology. J Immunol Methods 18:165–182

    Google Scholar 

  32. Timpl R, Glanville RW, Wick G, Martin GR (1979) Immunochemical study on basement membrane (type IV) collagens. Immunology 38:109–116

    Google Scholar 

  33. Tsukamoto Y, Helsel WE, Wahl SM (1981) Macrophage production of fibronectin a chemoattractant for fibroblasts. J Immunol 127:673–678

    Google Scholar 

  34. Turksen K, Aubin JE, Sodek J, Kalnins VI (1984) Changes in distribution of laminin, fibronectin, type IV collagen and heparin sulfate proteoglycan during colony formation by chick retinal pigment epithelial cells in vitro. Coll Relat Res 4:413–426

    Google Scholar 

  35. Turksen K, Aubin JE, Sodek J, Kalnins VI (1985) Localization of laminin, type IV collagen, fibronectin and heparin sulfate proteoglycan in chick retinal pigment epithelium basement membrane during embryonic development. J Histochem Cytochem 33:665–671

    Google Scholar 

  36. Wallow IHL, Miller SA (1978) Preretinal membrane by retinal pigment epithelium. Arch Ophthalmol 96:1643–1646

    Google Scholar 

  37. Wang K, Feramisco JR, Ash JF (1982) Fluorescent localization of contractile proteins in tissue culture cells. In: Frederiksen DW, Cunningham LW (eds) Methods in enzymology, vol 85. Academic Press, New York London Paris, pp 514–562

    Google Scholar 

  38. Wick G, Glanville RW, Timpl R (1979) Characterization of antibodies to basement membrane (type IV) collagen in immunohistological studies. Immunobiology 156:372–381

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Universitäts-Augenklinik, Mathildenstrasse 8, D-8000, München 2, Federal Republic of Germany

    O. F. Scheiffarth, A. Kampik & H. Günther

  2. Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany

    K. von der Mark

Authors
  1. O. F. Scheiffarth
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Kampik
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Günther
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. von der Mark
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Scheiffarth, O.F., Kampik, A., Günther, H. et al. Proteins of the extracellular matrix in vitreoretinal membranes. Graefe's Arch Clin Exp Ophthalmol 226, 357–361 (1988). https://doi.org/10.1007/BF02172967

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02172967

Keywords

Search

Navigation