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Evaluation of the endothelium of human donor corneas by induced dilation of intercellular spaces and trypan blue

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Abstract

The endothelium of 30 pairs of human cadaver corneas was stained by trypan blue and the intercellular spaces were visualized by induced dilation prior to corneal culture. Trypan blue staining and induced dilation of intercellular spaces by 0.9% and 0.45% NaCl were found to be atraumatic. Only a fraction of damaged cells were stained by trypan blue. Endothelial cell losses in culture did not correlate with the number of trypan-blue stained cells, the post-mortem time, or donor age.

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References

  • Aquavella JV, Van Horn DL, Haggerty CJ (1975) Corneal preservation using M-K medium. Am J Ophthalmol 80:791–799

    Google Scholar 

  • Bigar F, Schimmelpfennig BH, Gieseler R (1976) Routine evaluation of endothelium in human donor corneas. Graefe's Arch Clin Exp Ophthalmol 200:195–200

    Google Scholar 

  • Bourne WM, Doughman DJ, Lindstrom RL, Kolb MJ, Mindrup B, Skelnik D (1983) Increased endothelial cell loss after transplantation of corneas preserved by a modified organ culture technique. Ophthalmology [Suppl] A.R.V.O. abstracts

  • Campbell RC, Bourne WM, Campell RJ (1978) Endothelial changes in rabbit corneas. Arch Ophthalmol 96:2108–2110

    Google Scholar 

  • Fernandez-Meijide RE, Troncoso VV, Malbran ES (1970) Determination de la viabilidad endotelial en las queratoplastias perforantes. Arch Ophthalmol (Buinos Aires) 45:143–152

    Google Scholar 

  • Hassard DTR (1965) Selection of donor material for corneal grafting. In: King JH Jr., McTigue JW (eds) The Cornea World Congress. Butterworths, London, pp 370–383

    Google Scholar 

  • Hoefle FB (1969) Human corneal donor material. Arch Ophthalmol 82:361–367

    Google Scholar 

  • Jans RG, Hassard DTR (1967) Lissamine green. A supravital stain for determination of corneal endothelial viability. Can J Ophthalmol 2:297–302

    Google Scholar 

  • Jensen OA, Prause JU, Laursen H (1981) Shrinkage in preparatory steps for SEM. A study on rabbit corneal endothelium. Graefe's Arch Clin Exp Ophthalmol 215:233–342

    Google Scholar 

  • Kirk AH, Hassard DTR (1969) Supravital staining of the corneal endothelium and evidence for a membrane on its surface. Can J Ophthalmol 4:405–415

    Google Scholar 

  • Krachmer JH, Schnitzer JI, Fratkin J (1981) Cornea pseudogutata. A clinical and histopathologic description of endothelial cell edema. Arch Ophthalmol 99:1377–1381

    Google Scholar 

  • Neubauer L, Laing RA, Leibowitz HM (1984) Specular microscopic appearance of damaged and dead endothelial cells in corneas following short-term storage. Arch Ophthalmol 120:439–444

    Google Scholar 

  • Norn MS (1980) Per operative trypan blue vital staining of corneal endothelium. Eight years follow up. Acta Opthalmol (Copenh) 58:550–555

    Google Scholar 

  • Olsen T (1981) Transient changes in specular appearance of the corneal endothelium and in corneal thickness during anterior uveitis. Acta Ophthalmol (Copenh) 59:100–109

    Google Scholar 

  • Sperling S (1977) Combined staining of corneal endothelium by alizarine red and trypan blue. Acta Ophthalmol (Copenh) 55:1–8

    Google Scholar 

  • Sperling S (1981) Cryopreservation of human cadaver corneas regenerated at 31° C in a modified tissue culture medium. Acta Ophthalmol (Copenh) 59:142–148

    Google Scholar 

  • Sperling S (1985) Assessment of endothelial cell density in bovine corneas after osmotically induced dilation of intercellular spaces. Cornea 4:71–79

    Google Scholar 

  • Sperling S (1986) Estimates of endothelial cell densities in whole human eyes and in excised corneas. Cornea vol 5 (in press)

  • Sperling S, Gundersen HJG (1978) The precision of unbiased estimates of numerical density of endothelial cells in donor corneas. Acta Ophthalmol (Copenh) 56:793–802

    Google Scholar 

  • Sperling S, Sørensen I (1981) Decontamination of cadaver corneas. Acta Ophthalmol (Copenh) 59:126–133

    Google Scholar 

  • Sperling S, Olsen T, Ehlers N (1981) Fresh and cultured corneal grafts compared by post-operative thickness and endothelial cell density. Acta Ophthalmol (Copenh) 59:566–575

    Google Scholar 

  • Stocker FW, King EH, Lucas DO, Georgiade NA (1970) Clinical test for evaluating donor corneas. Arch Ophthalmol 84:2–7

    Google Scholar 

  • Van Horn DL, Schultz DR, DeBruin J (1975) Endothelial survival in corneal tissue stored in M-K Medium. Am J Ophthalmol 80:642–647

    Google Scholar 

  • Wickham MG, Binder PS (1976) Evaluation of corneal endothelial damage using correlated microscopy techniques. Ophthalmic Res 8:407–413

    Google Scholar 

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Authors and Affiliations

  1. University of Aarhus, Aarhus, Denmark

    Steffen Sperling

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  1. Steffen Sperling
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Sperling, S. Evaluation of the endothelium of human donor corneas by induced dilation of intercellular spaces and trypan blue. Graefe's Arch Clin Exp Ophthalmol 224, 428–434 (1986). https://doi.org/10.1007/BF02173358

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  • DOI: https://doi.org/10.1007/BF02173358

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