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Distribution of p27(KIP1), cyclin D1, and proliferating cell nuclear antigen after retinal detachment

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Abstract

Purpose

To examine the expression of the p27(KIP1), cyclin D1, and proliferating cell nuclear antigen (PCNA) in the retina and retinal pigment epithelium (RPE) after retinal detachment.

Methods

Normal eyes and eyes at 2 or 4 days after retinal detachment with the C57B16 mouse were analyzed by immunocytochemistry using anti-p27(KIP1), anti-cyclin D1, and anti-proliferating cell nuclear antigen (PCNA) antibodies as well as anti-glutamate synthetase (GS) antibody.

Results

The p27(KIP1) positive nuclei were distributed in the inner nuclear layer (INL) and the RPE of the normal mice eye. In the INL, p27(KIP1) was detected in the middle sublayer, where the nuclei of glutamate synthetase positive Müller cells were situated. In contrast, cyclin D1 was not detected either in the retina or in the RPE. At 2 and 4 days after the retinal detachment, RPE cells under the detached retina were negative for p27(KIP1) and positive for cyclin D1 and PCNA. In the INL of the detached retina, p27(KIP1) was detected after 2 days, but was not detected after 4 days. In contrast, PCNA was not detected in the INL after 2 days, but was detected after 4 days. Cyclin D1 was detected in the middle sublayer of the INL at both 2 and 4 days after the retinal detachment.

Conclusion

These results suggested that degradation of p27(KIP1) and expression of cyclin D1 was involved in the proliferation of the Müller cells as well as RPE cells after retinal detachment.

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Author notes

  1. Kazuhiko Yoshida

    Present address: Department of Ophthalmology, Hokkaido University School of Medicine, N17 W5, 060-8638, Sapporo, Japan

Authors and Affiliations

  1. Department of Ophthalmology, Hokkaido University School of Medicine, N15 W7, Kita-ku, 060-8638, Sapporo, Japan

    Kazuhiko Yoshida, Satoru Kase, Takayuki Harada, Chikako Harada, Kazuhiro Ohgami, Kenji Shiratori & Shigeaki Ohno

  2. Department of Molecular Neurobiology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, Tokyo 183–8526, Japan

    Takayuki Harada & Chikako Harada

  3. Department of Biochemistry, Hokkaido University School of Medicine, Sapporo, Japan

    Hiromi Ikeda

  4. Department of Anatomy, Nippon Medical College, Sendagi, Bunkyo-ku, Tokyo, Japan

    Junko Imaki

  5. Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan

    Keiko Nakayama, Hiroyasu Nagahama & Keiichi I. Nakayama

  6. Division of Developmental Genetics, Center for Translational and Advanced Animal Research on Human Diseases, Tohoku University Graduate School of Medicine, Miyagi, Japan

    Keiko Nakayama

Authors
  1. Kazuhiko Yoshida
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  2. Satoru Kase
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  9. Kazuhiro Ohgami
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Correspondence to Kazuhiko Yoshida.

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Yoshida, K., Kase, S., Nakayama, K. et al. Distribution of p27(KIP1), cyclin D1, and proliferating cell nuclear antigen after retinal detachment. Graefe's Arch Clin Exp Ophthalmol 242, 437–441 (2004). https://doi.org/10.1007/s00417-004-0861-7

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  • DOI: https://doi.org/10.1007/s00417-004-0861-7

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