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Genetic variations in the gB, UL144 and UL149 genes of human cytomegalovirus strains collected from congenitally and postnatally infected Japanese children

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Abstract

Human cytomegalovirus (CMV) is the leading cause of intrauterine viral infection. The association of genetic polymorphisms in some particular genes with the incidence and severity of congenital infection has been controversial. To address this issue, we analyzed the genotypes of the glycoprotein B (gB), UL144 and UL149 genes of CMV clinical strains obtained from 33 congenitally and 31 postnatally infected Japanese children. Our results demonstrated that (1) CMV strains with any combination of genotypes could be vertically transmitted from mother to fetus, potentially causing neurological abnormalities, (2) the gB3 genotype was more prevalent in the congenital cases than in postnatally infected children (P < 0.05), particularly in congenital cases with sensorineural hearing loss (P = 0.009), (3) there was no relationship between gB genotype and viral load in the urine and dried umbilical cord specimens in the congenital cases, and (4) the UL144 and UL149 genotype distributions had no bias for congenial infection. In future studies, it would be interesting to see whether the gB genotypes serve as a prognostic indicator of CMV-associated diseases.

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Abbreviations

CMV:

Cytomegalovirus

PCR:

Polymerase chain reaction

SNHL:

Sensorineural hearing loss

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Acknowledgments

We thank Phillip E. Pellett, Naoki Nozawa, and Kenji Fujieda for their intellectual input, and Risa Taniguchi and Hitomi Komura for their technical assistance. This study was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan, a Grant for Child Health and Development from the Ministry of Health and Welfare, Japan (NI), and a fellowship from the Atsumi Foundation (HY).

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Authors and Affiliations

  1. Department of Virology I, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan

    Hainian Yan, Yuhki Inami, Yumiko Yamamoto, Ichiro Kurane & Naoki Inoue

  2. Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan

    Hainian Yan, Masashi Mizuguchi & Hiroshi Ushijima

  3. Department of Pediatrics, Asahikawa Medical College, Hokkaido, Japan

    Shin Koyano

  4. Department of Microbiology, Fukushima Medical University, Fukushima, Japan

    Tatsuo Suzutani

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  1. Hainian Yan
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Correspondence to Naoki Inoue.

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Yan, H., Koyano, S., Inami, Y. et al. Genetic variations in the gB, UL144 and UL149 genes of human cytomegalovirus strains collected from congenitally and postnatally infected Japanese children. Arch Virol 153, 667–674 (2008). https://doi.org/10.1007/s00705-008-0044-7

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