ELISA for the core protein of the cartilage large aggregating proteoglycan, aggrecan: Comparison with the concentrations of immunogenic keratan sulphate in synovial fluid, serum and urine
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2018, Molecular Genetics and MetabolismConcentrations of synovial fluid biomarkers and the prediction of knee osteoarthritis 16 years after anterior cruciate ligament injury
2017, Osteoarthritis and CartilageCitation Excerpt :Knee-joint fluid samples were aspirated and centrifuged at 3000 ×g, and aliquots of the supernatants were frozen and stored at −70°C within a few hours of collection. Aggrecan, using monoclonal antibody 1-F215,32; COMP, commercially available assay from AnaMar; MMP-3 and TIMP-1 were quantified by enzyme-linked immunoassays as described5,17,33,34. At baseline, there were no signs of radiological OA in any of the ACL-injured subjects.
Glycosaminoglycans detection methods: Applications of mass spectrometry
2017, Molecular Genetics and MetabolismCitation Excerpt :There are different types of ELISA: direct [114], indirect [115], sandwich [116], competitive [117]. ELISA assays developed for GAGs can measure levels of KS [118–120], HS [121–122], C4S [123], C6S [123], DS [123], and hyaluronic acid [124]. ELISA protocols have also been developed to detect GAGs in cells and on cell-surfaces [125–126].
An ARGS-aggrecan assay for analysis in blood and synovial fluid
2014, Osteoarthritis and Cartilage