Association between mast cells and the development of experimental autoimmune uveitis in different rat strains

doi:10.1016/0090-1229(92)90160-P

Clinical Immunology and Immunopathology

Volume 65, Issue 3, December 1992, Pages 294-299

Clinical Immunology ...

https://doi.org/10.1016/0090-1229(92)90160-P Get rights and content

Abstract

To study the role of anterior uveal mast cells in experimental autoimmune uveitis (EAU), the mast cells in the iris and ciliary body of Lewis rats, Brown Norway (BN) rats, and their F1 hybrids (LBNF1) were quantitated in normal rats and during the induction period of EAU. The mean baseline mast cell number was 68.9 ± 10.8 per anterior uvea for Lewis rats, 0.3 ± 0.2 for BN rats, and 4.6 ± 0.6 for LBNF1 rats. Detectable mast cells in the anterior uvea of S-Ag-immunized Lewis rats decreased to 60% of control at 6 days postimmunization, recovered to 80% at 10 days, and dropped again to 16% at 13 days, with disease onset around 14 days. In Lewis rats that were adoptively transferred with a uveitogenic T-lymphocyte line, a profound drop in anterior uveal mast cell numbers occurred in the eyes with early signs of EAU, 3 days after the transfer. The decrease in detectable mast cells is consistent with mast cell degranulation. The data suggest that anterior mast cells participate in the immunopathogenesis of EAU and may influence the genetic susceptibility to EAU.

References (29)

N.C. Stanley et al.
Attenuation of experimental autoimmune encephalomyelitis by compound 48/80 in Lewis rats
J. Neuroimmunol.
(1990)
L. Bø et al.
Mast cells in brains during experimental allergic encephalomyelitis in Lewis rats
J. Neurol. Sci.
(1991)
D.S. Linthicum
Development of acute autoimmune encephalomyelitis in mice: Factors regulating the effector phase of the disease
Immunobiology
(1982)
R.R. Caspi et al.
T cell lines mediating experimental autoimmune uveoretinitis (EAU) in the rat
J. Immunol.
(1986)
H. Sanui et al.
Identification of an immunodominant and highly immunopathogenic determinant in the retinal interphotoreceptor retinoid-binding protein (IRBP)
J. Exp. Med.
(1989)
Y. de Kozak et al.
S antigen-induced experimental autoimmune uveo-retinitis in rats
Curr. Eye Res.
(1981)
I. Gery et al.
Differences in susceptibility to experimental autoimmune uveitis among rats of various strains
M. Mochizuki et al.
An association between susceptibility to experimental autoimmune uveitis and choroidal mast cell numbers
J. Immunol.
(1984)
Y. de Kozak et al.
Pharmacological modulation of IgE-dependent mast cell degranulation in experimental autoimmune uveoretinitis
Jpn. J. Ophthalmol.
(1983)

Y. de Kozak et al.

Evidence for immediate hypersensitivity phenomena in experimental autoimmune uveoretinitis

Eur. J. Immunol.

(1981)

S.J. Galli et al.

Basophils and mast cells: Morphologic insights into their biology, secretory patterns, and function

Prog. Allergy

(1984)

M. Plaut et al.

Mast cell lines produce lymphokines in response to cross-linkage of FceRI or to calcium ionophores

Nature

(1989)

Marshall, J. S., and Bienenstock, J., Mast cells. Springer Semin. Immunopathol., 12,...

Cited by (18)

Rat immunology
2019, The Laboratory Rat
The immune system plays a primary role in mechanisms of defense against disease and the maintenance of homeostasis for all species, including rats. With this chapter, we hope to highlight significant contributions from the rat to the field of immunology and provide a useful reference for non-immunologists who may use rat models in research. The reader is referred to more comprehensive immunology and laboratory animal textbooks for additional background on information throughout this chapter. This chapter provides a wide overview of important attributes and contributions for the rat in immunology and functions as a reference to enhance further exploration of rat biological models.
Ocular Mast Cells and Mediators
2008, Immunology and Allergy Clinics of North America
Citation Excerpt :
In this animal model of human autoimmune ocular diseases, an intense bilateral panophthalmic inflammation develops 10 to 18 days after inoculation with the retinal S-antigen emulsified in an appropriate adjuvant, or 3 days after adoptive transfer with a uveitogenic T-lymphocyte line. Several studies have documented an association between baseline mast cell numbers in the anterior uvea and choroid, and the susceptibility to experimental autoimmune uveitis (EAU) [111,114,115]. Two inbred rat strains, CAR and Lewis, are high responders to the induction of EAU, and essentially all animals develop the disorders postimmunization with retinal antigens.
Mast cells have long been recognized for their role in immediate hypersensitivity reactions, by virtue of the presence of high affinity receptors for IgE (FcɛRI) on their surface. More recently, mast cells have been postulated to be involved in a variety of chronic inflammatory disorders as numerous mediators released by activated mast cells are characterized. This article summarizes current information on mast cell mediators, heterogeneity, and differentiation, and it reviews studies of mast cells in the normal eye and various ocular disorders.
Strain differences in histamine release from peritoneal mast cells in rats
1998, General Pharmacology
- 1.
  Peritoneal mast cells from Brown-Norway (BN) rats were compared with those from Wistar and Sprague-Dawley (SD) rats.
- 2.
  Peritoneal mast cells from BN rats showed the smallest values in number, cell diameter and histamine contents compared with those from Wistar and SD rats.
- 3.
  BN rat peritoneal mast cells were more sensitive to compound 48/80 and anti-IgE than were those from Wistar and SD rats, and they showed a higher response to A23187 than did cells from Wistar rats.
- 4.
  The histamine release from passively sensitized peritoneal mast cells was weaker in BN rats than was that from Wistar and SD rats.
Ocular mast cells and mediators
1997, Immunology and Allergy Clinics of North America
Mast cells have long been recognized for their role in immediate hypersensitivity reactions, by virtue of the presence of high affinity receptors for IgE (FcεRI) on their surface. More recently, mast cells have been postulated to be involved in a variety of chronic inflammatory disorders as numerous mediators released by activated mast cells are characterized. This article summarizes current information on mast cell mediators, heterogeneity, and differentiation, and reviews studies of mast cells in the normal eye as well as in various ocular disorders.
Experimental autoimmune uveitis and its relationship to clinical ocular inflammatory disease
1996, Journal of Autoimmunity
Mast cells in accessory glands of experimentally induced prostatitis in male wistar rats
1995, Clinical Immunology and Immunopathology
We studied the histological modificatious in the accessory glands of autoimmune rats. Adult male Wistar rats were id immunized three times with saline extract of rat male accessory glands (RAG) chemically modified (MRAG) in complete Freund's adjuvaut (CFA) (groups 1, 2, and 3). Prior to the first immunization with MRAG-CFA groups 2 and 3 received peritoneal cells obtained from rats that had been injected 2 or 24 hr previously with low doses of RAG. Furthermore, an additional group (group 4) ip immunized with liposome-associated-RAG was incorporated. The delayed-type hypersensitivity response studied 10 or 15 days after first immunization was positive for rats of groups 1, 3, and 4, while it was negative for rats of group 2. Serum samples obtained on Day 45 and studied by ELISA showed high levels of autoantibodies in groups 1 and 2 and lower levels of autoantibodies in group 3, but did not show autoantibodies in group 4. The histological studies performed 10 days after the last immunization showed organ-specific lesions in the accessory glands in animals of groups 1, 3, and 4. Infiltration of mononuclear cells was the main alteration in group 1, while infiltration of mast cells and polymorphonuclear leukocytes were present in specimens of groups 3 and 4. The main finding of this study was a significant increase (P < 0.0005) in the extent of mast cell degranulation in the specimens of accessory glands stained with toluidine blue. Our results suggest that mast cells are activated in our experimental model of autoimmune disease.

View all citing articles on Scopus

View full text

Regular articleAssociation between mast cells and the development of experimental autoimmune uveitis in different rat strains

Abstract

J. Neuroimmunol.

J. Neurol. Sci.

Immunobiology

T cell lines mediating experimental autoimmune uveoretinitis (EAU) in the rat

J. Immunol.

Identification of an immunodominant and highly immunopathogenic determinant in the retinal interphotoreceptor retinoid-binding protein (IRBP)

J. Exp. Med.

S antigen-induced experimental autoimmune uveo-retinitis in rats

Curr. Eye Res.

Differences in susceptibility to experimental autoimmune uveitis among rats of various strains

An association between susceptibility to experimental autoimmune uveitis and choroidal mast cell numbers