Clinical Transplantation ProceedingsAcceptance of Second Corneal Allograft by Combination of Anti-VLA-4 and Anti-LFA-1 Monoclonal Antibodies in Mice☆
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MAbs and Animals
Hybridomas producing PS/2 (rat IgG2b)[3]and KBA (α chain of LFA-1, anti-CD11a, IgG2a)[4]that react with murine VLA-4 and LFA-1, respectively, were kindly provided by Dr K. Okumura (Juntendo University, Tokyo, Japan). The mAbs were purified from ascites by protein G column. Inbred strains of BALB/c (H-2d) and C3H/He (H-2k) male mice weighing 25 to 30 g (8 to 12 weeks old) were obtained from Clea Japan Co. (Tokyo, Japan).
Orthotopic Corneal Transplantation: First Graft
BALB/c mice were used as recipients and C3H/He mice were used as donors for
Results
Survival weeks of the first and second corneal allografts after challenge tests are shown in Table 1. Three of five mice accepted the second corneal allografts for eight weeks; at that time, four of five first corneal grafts still remained clear (14 weeks after first grafting).
Conclusion
The challenge test is a most reliable method to evaluate immunologic tolerance. In the present study, 60% of mice accepted both the first and the second grafts for more than eight weeks after the second grafting (more than 14 weeks after the first grafting). He et al.[5]reported that second corneal allografts were not accepted by the combined use of anti–LFA-1 and anti-intercellular adhesion molecule (ICAM)-1 mAbs in mice. In contrast, in the present study, with donor-syngeneic second corneal
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Cited by (5)
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1999, British Journal of Ophthalmology
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This study was Supported in part by a grant from the Ministry of Education, Science and Culture, Japan (B 06454495).