Effect of chemical structure of hydrogels on the adhesion and phenotypic characteristics of human monocytes such as expression of galectins and other carbohydrate-binding sites

doi:10.1016/S0142-9612(97)00037-9

Biomaterials

Volume 18, Issue 14, July 1997, Pages 1009-1014

https://doi.org/10.1016/S0142-9612(97)00037-9 Get rights and content

Abstract

The reactivity of diverse immune aspects to the presence of synthetic polymers represents one of the most important aspects of implantable device biocompatibility. In this report, we show the effect of the chemical structure of a synthetic polymer support on monocyte adhesion and selected phenotypic characteristics in vitro as a model for the initial steps of non-self-recognition of an implant. The extent of monocyte adhesion was significantly influenced by the support chemistry. The highest level of monocyte adhesion was observed on a surface copolymer of 2-hydroxyethyl methacrylate with dimethyl aminoethyl methacrylate relative to results of experiments in which poly(2-hydroxyethyl methacrylate) or the copolymer of 2-hydroxyethyl methacrylate with the sodium salt of methacrylic acid was used. Cell adhesion to the polymers tested and to glass was accompanied by enhanced expression of the carbohydrate-binding sites tested for asialoglycoprotein β-galactosides such as galectins, β-N-acetylgalactosamine, α-mannoside, specific lectin for heparin as well as the lymphokine-macrophage migration inhibitory factor in the monocytes tested. These results suggest the importance of monocyte adhesion to the biomaterial surface for their development into macrophages and further non-self-recognition of the implanted device.

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^‡: F.-T. Liu presently at La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA.

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Effect of chemical structure of hydrogels on the adhesion and phenotypic characteristics of human monocytes such as expression of galectins and other carbohydrate-binding sites

Abstract

Biomaterials

J. Invest. Dermatol.

Biomaterials

Exp. Mol. Pathol.

Clin. Mater.

Arch. Biochem. Biophys.

Biochem. Biophys. Res. Commun.

Biomaterials

Biomaterials

Eur. J. Cancer

Fibrin(ogen) mediates acute inflammatory responses

J. Exp. Med.

Complement C3 participation in monocyte adhesion to different surfaces

Mechanism of acute inflammatory response to biomaterials

Cell Mater.

Fibrin enhances the expression of IL-1β by human peripheral blood mononuclear cells

J. Immunol.

Inflammatory responses to biomaterials

Am. J. Pathol.

Functional versus quantitative comparison of IL-1β from monocytes/macrophages on biomedical polymers

J. Biomed. Mater. Res.

Protein adsorption and macrophage activation on polydimethylsiloxane and silicone rubber

J. Biomater. Sci. (Polymer Edn)

Role for interleukin-4 in foreign-body giant cell formation on a poly(etherurethane urea) in vivo

J. Biomed. Mater. Res.

Interleukin-4 induces foreign body giant cells from human monocytes/macrophages

Am. J. Pathol.