Original ArticlesExpansion of γδ T-cells in Behçet's disease: Role of disease activity and microbial flora in oral ulcers☆,☆☆
Section snippets
Determination of T-cell antigens
PBMCs were isolated from heparinized blood by means of Ficoll-Hypaque (Sigma Chemical, St. Louis, Mo) density centrifugation.16 To detect surface-membrane antigens, we spun down 0.5 to 1 × 106 cells in 5-mL glass tubes in PBS solution (pH 7.4). Resuspended pellets were stained directly with mAb directly conjugated with fluorochromes or with saturating concentrations of primary murine mAb at 4°C for 30 minutes and washed twice in PBS and 0.2% sodium azide. FITC-conjugated goat anti-mouse Ig
T-cell subsets in PB
We compared the percentages of CD3+, CD8+, and γδ TCR+ cells in the PBMCs of patients with BD (n = 23) and with FMF (n = 20), as well as in the normal controls (n = 18). As shown in Fig 1, CD3+ cells constituted 60.58% ± 16.15%, 56.93% ± 14.7%, and 60.34% ± 14.89% of the PBMCs, respectively, whereas CD8+ cells accounted for 25.6% ± 8.01%, 23.1% ± 8.6%, and 21.9% ± 12.8% of the PBMCs, respectively.
Discussion
The results of these experiments confirm and expand those reported from Japan, Turkey, and Great Britain with regard to activation of the γδ T-cell subset in BD.19, 20, 21, 22 Thus a significant increase in the percentage of these cells among the PBMCs of Israeli BD patients relative to both normal individuals and patients with FMF was detected. Moreover, our results demonstrate that the increase in γδ T-cells is related to disease activity and that products of microorganisms from oral
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2018, Autoimmunity ReviewsCitation Excerpt :γδ T lymphocytes act as the first line of host defence in mucosal immunity. An increased proportion of activated γδ T cells has been shown [48,49] in BD, suggesting a role for them in the pathogenesis of the disease [50,51]. Furthermore, accumulation of γδ T cells in the sites of inflammation in BD has been reported [52].
Epigenetic alterations in chronic disease focusing on Behçet's disease: Review
2017, Biomedicine and PharmacotherapyGenetics of Behçet disease
2016, Medicina ClinicaPulchinenoside B4 ameliorates oral ulcers in rats by modulating gut microbiota and metabolites
2024, Applied Microbiology and Biotechnology
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Reprint requests: Ilan Bank, MD, Department of Medicine, Chaim Sheba Medical Center, Tel Hashomer 52621, Israel; e-mail: [email protected].
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0022-2143/2003 $30.00 + 0