Abstract
The expression of two factors involved in the nuclear-mitochondrial crosstalk, namely the mitochondrial transcription factor A (TFAM) and the nuclear respiratory factor-1 (NRF-1), was studied in human skeletal muscle biopsies of young and aged subjects. Aged subjects presented a 2.6-fold and an 11-fold increase of the levels of TFAM protein and TFAM mRNA, respectively. The increased expression of TFAM was associated to the doubling of NRF-1 DNA-binding affinity and to a 6-fold increase of NRF-1 mRNA level. The upregulation of TFAM and NRF-1, in aged skeletal muscle, appears involved in the pathway leading to the age-related increase of mitochondrial DNA content.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Aged, 80 and over
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Aging / physiology*
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Biopsy
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Gene Expression*
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Humans
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Mitochondria / genetics
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Mitochondria / metabolism
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Mitochondrial Proteins*
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Muscle, Skeletal / cytology
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Muscle, Skeletal / metabolism*
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NF-E2-Related Factor 1
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Nuclear Respiratory Factor 1
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Nuclear Respiratory Factors
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Protein Binding
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Trans-Activators / genetics
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Trans-Activators / metabolism*
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Transcription Factors / genetics
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Transcription Factors / metabolism*
Substances
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DNA-Binding Proteins
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Mitochondrial Proteins
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NF-E2-Related Factor 1
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NRF1 protein, human
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Nuclear Proteins
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Nuclear Respiratory Factor 1
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Nuclear Respiratory Factors
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RNA, Messenger
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TFAM protein, human
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Trans-Activators
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Transcription Factors
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mitochondrial transcription factor A