An optimized protocol for first strand cDNA synthesis from laser capture microdissected tissue

Lab Invest. 2001 Aug;81(8):1167-9. doi: 10.1038/labinvest.3780329.
No abstract available

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • DNA, Complementary / biosynthesis*
  • Dissection / methods*
  • Glyceraldehyde-3-Phosphate Dehydrogenases / biosynthesis
  • Glyceraldehyde-3-Phosphate Dehydrogenases / genetics
  • Humans
  • Lasers*
  • Macular Degeneration / genetics
  • Macular Degeneration / metabolism*
  • Macular Degeneration / pathology
  • Middle Aged
  • Pigment Epithelium of Eye / metabolism
  • Pigment Epithelium of Eye / pathology
  • Polymerase Chain Reaction / methods*
  • RNA, Messenger / biosynthesis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sensitivity and Specificity

Substances

  • DNA, Complementary
  • RNA, Messenger
  • Glyceraldehyde-3-Phosphate Dehydrogenases