Abstract
Purpose:
To examine the involvement of the c-maf gene in the proliferation of the lens cells.
Methods:
Eyes of the E13 and E18 stages of the wild-type and c-maf-/- mice were analyzed by BrdU incorporation assay, TUNEL assay and immunocytochemistry using a anti-P27(KIP1) and a anti-P57(KIP2) antibody.
Results:
In the E13 and E18 c-maf mutant lens, BrdU-positive cells were detected at the posterior region of the lens. Cell-cycle inhibitor P27(KIP1) and P57(KIP2) were expressed in the equatorial and posterior region of the lens of both wild-type and c-maf-/- lenses.
Conclusion:
These results suggest that the expression of c-maf is required for differentiation and cell cycle arrest of lens fiber cells. It is also suggested that P27(KIP1) and P57(KIP2) were not involved in the continued proliferation of posterior region of the c-maf-/- lens.
MeSH terms
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Animals
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Apoptosis / physiology
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Bromodeoxyuridine
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Cell Cycle
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Cell Cycle Proteins / metabolism
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Cell Differentiation
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Cell Division / physiology
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Cyclin-Dependent Kinase Inhibitor p27
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Cyclin-Dependent Kinase Inhibitor p57
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DNA / biosynthesis
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DNA-Binding Proteins / physiology*
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Female
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Fluorescent Antibody Technique, Indirect
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In Situ Nick-End Labeling
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Lens, Crystalline / cytology*
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Lens, Crystalline / metabolism
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Mice
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Mice, Inbred DBA
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Mice, Knockout
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Nuclear Proteins / metabolism
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Pregnancy
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Proto-Oncogene Proteins / physiology*
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Proto-Oncogene Proteins c-maf
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Tumor Suppressor Proteins / metabolism
Substances
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Cdkn1b protein, mouse
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Cdkn1c protein, mouse
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Cell Cycle Proteins
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Cyclin-Dependent Kinase Inhibitor p57
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DNA-Binding Proteins
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Maf protein, mouse
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Nuclear Proteins
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-maf
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Tumor Suppressor Proteins
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Cyclin-Dependent Kinase Inhibitor p27
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DNA
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Bromodeoxyuridine