Abstract
This study was aimed to investigate the expression of glutamate receptors and calcium-binding proteins in 1- and 4-month/s (mo) streptozotocin (STZ)-induced diabetic rats. Upregulation of glutamate receptors' [N-methyl-D-aspartate receptor (NMDAR)1 and GluR2/3] immunoreactivities was observed in the ganglion, amacrine and bipolar cells as well as in the inner and outer plexiform layers (IPL and OPL) in 1 mo diabetes and was further enhanced at 4 mo. Immunoreactivity of calcium-binding proteins (calbindin and parvalbumin) was also concomitantly increased. The present results suggest that upregulation of glutamate receptors and calcium-binding proteins may reflect changes of the glutamate and calcium metabolism in the diabetic retina. It is speculated that the above changes in the IPL and OPL may be linked to alteration of synaptic transmission in the diabetic retina.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amacrine Cells / metabolism
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Amacrine Cells / pathology
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Animals
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Calbindins
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Calcium-Binding Proteins / metabolism*
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Diabetes Mellitus, Experimental / complications*
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Diabetic Retinopathy / metabolism*
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Diabetic Retinopathy / pathology
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Diabetic Retinopathy / physiopathology
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Disease Models, Animal
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Glutamic Acid / metabolism
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Immunohistochemistry
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Male
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Nerve Degeneration / metabolism*
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Nerve Degeneration / pathology
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Nerve Degeneration / physiopathology
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Neurons / metabolism
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Neurons / pathology
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Parvalbumins / metabolism
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Rats
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Rats, Wistar
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Receptors, AMPA / metabolism
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Receptors, Glutamate / metabolism*
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Receptors, N-Methyl-D-Aspartate / metabolism
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Retina / metabolism*
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Retina / pathology
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Retinal Ganglion Cells / metabolism
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Retinal Ganglion Cells / pathology
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S100 Calcium Binding Protein G / metabolism
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Synaptic Transmission / physiology
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Up-Regulation / physiology
Substances
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Calbindins
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Calcium-Binding Proteins
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NMDA receptor A1
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Parvalbumins
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Receptors, AMPA
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Receptors, Glutamate
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Receptors, N-Methyl-D-Aspartate
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S100 Calcium Binding Protein G
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Glutamic Acid
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glutamate receptor ionotropic, AMPA 2