We studied the rate of contamination in the contact lens care systems and conjunctivas of 30 asymptomatic cosmetic soft contact lens wearers. In caring for their lenses, 23 patients used chemical disinfection systems (quaternary ammonium solutions), six patients used hydrogen peroxide, and one used heat. Eighteen patients used unpreserved intravenous saline solution, and only seven patients used commercially prepared preserved saline solutions. Cultures were obtained from lens cases, lens care solutions, and conjunctivas. Contamination was found in 70% of the lens care systems. Fifty-seven percent of the patients had lens case contamination, and 17% had conjunctival contamination. The most frequently isolated bacteria in the lens care systems were Pseudomonas, Staphylococcus epidermidis, and Escherichia coli. We suggest the development of more effective systems for disinfection.