Variations in ascorbate and thiol concentration in lens and aqueous humour, with age and vitamin C nutrition, are of potential biological importance. To study these relationships, Dunkin-Hartley guinea-pigs were maintained for periods of up to 1.3 yr on diets containing either high or low (marginal) vitamin C. Ascorbate contents of liver, spleen, adrenals, lens and aqueous humour, and acid-soluble thiol of lens and aqueous humour were measured. High vitamin C intake maintained ascorbate levels in the internal organs between five and 30 times the level attained by the low vitamin C intake and aqueous humour vitamin C was 10-20 times higher at high vitamin C intake. Lens ascorbate, however, was only about twice as high at high vitamin C intake than at low intake, and at both intake levels it declined steadily to about half its initial value, after 1.3 yr. Thus an animal aged 1.3 yr on the low intake had about one-quarter to one-fifth of the lens ascorbate level of a young animal receiving a generous intake. Acid-soluble thiol in the lens, in contrast to ascorbic acid, increased significantly with age but was not significantly affected by dietary vitamin C intake. Acid-soluble thiol in the aqueous humour was only 0.5-2% of the concentration found in lens, and unlike the lens thiol level, it declined with age. No sex differences were observed for ascorbate or thiol levels either in lens or in aqueous humour.