Epilation, electrolysis, cryotherapy, and argon laser thermal ablation were used to remove eyelashes of rabbits. Epilation and electrolysis were done in the standard fashion. Nitrous oxide cryotherapy was applied in a double rapid-freeze/slow-thaw manner for either 30 or 60 seconds. Argon laser settings were 1 W, 50-micron spot size, and durations of 0.2, 0.5, or 1.0 second. The rabbits were euthanized immediately, 24 hours, 2 weeks, or 3 months after the procedure. Epilation was followed by total regrowth of eyelashes within 2 weeks. Electrolysis showed focal destruction of follicles with variable regrowth. Cryotherapy resulted in moderate-to-severe eyelid scarring with minimal regrowth. Argon laser thermal ablation produced focal necrosis and variable follicle destruction. Argon laser thermal ablation may be a suitable alternative to electrolysis or cryotherapy in selected cases of trichiasis.