Abstract
The present study demonstrates that human retinal pigmented epithelial cells produce nitric oxide (NO) upon co-treatment with interferon gamma (IFN gamma) and interleukin-1 beta (IL-1 beta). The biosynthesis of NO, which was measured by the accumulation of the stable end-product nitrite, requires an induction period of approximately 12 hours and continues for at least three days. The synthesis was abolished by the stereoselective inhibitors of NO synthase (NOS), NG-monomethyl-L-arginine, N omega-nitro-L-arginine and N omega-nitro-L-arginine methyl ester and by cycloheximide. Transforming growth factor beta suppressed cytokine-induced NOS. The results indicate that cytokines such as IFN gamma and IL-1 beta are capable of inducing NOS, while TGF beta prevents this induction, in subcultured pigmented epithelial cells from the human retina.
MeSH terms
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Amino Acid Oxidoreductases / antagonists & inhibitors
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Arginine / analogs & derivatives
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Arginine / pharmacology
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Cells, Cultured
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Cycloheximide / pharmacology
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Cytokines / pharmacology*
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Fibroblast Growth Factor 2 / pharmacology
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Humans
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Interferon-gamma / pharmacology
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Interleukin-1 / pharmacology
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Kinetics
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NG-Nitroarginine Methyl Ester
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Nitric Oxide / antagonists & inhibitors
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Nitric Oxide / biosynthesis*
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Nitric Oxide Synthase
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Nitrites / metabolism
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Nitroarginine
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Pigment Epithelium of Eye / drug effects
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Pigment Epithelium of Eye / metabolism*
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Recombinant Proteins / pharmacology
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Transforming Growth Factor beta / pharmacology
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Tumor Necrosis Factor-alpha / pharmacology
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omega-N-Methylarginine
Substances
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Cytokines
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Interleukin-1
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Nitrites
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Recombinant Proteins
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Transforming Growth Factor beta
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Tumor Necrosis Factor-alpha
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Fibroblast Growth Factor 2
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Nitroarginine
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omega-N-Methylarginine
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Nitric Oxide
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Interferon-gamma
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Arginine
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Cycloheximide
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Nitric Oxide Synthase
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Amino Acid Oxidoreductases
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NG-Nitroarginine Methyl Ester