We have refined a technique for performing 360 degrees trabeculotomy in a single procedure using a 6-0 polypropylene (Prolene) suture fragment and have used it in 15 patients (26 eyes) with primary congenital glaucoma. The refined technique can be performed via a single nasal surgical site. This procedure avoids many of the difficulties encountered with metal trabeculotomes and preserves conjunctiva should further glaucoma surgery be necessary.