Abstract
The effects of Concanavalin A on cell morphology, cytoskeleton arrangement and some metabolic activities of 11-day chick embryo fibroblasts were examined. In fibroblasts, Con A caused a dose-dependent round morphology and a change in tubulin, actin and alpha-actinin arrangement, whereas it did not modify 3H-thymidine incorporation. In addition, lectin stimulated more hyaluronic acid than sulphated glycosaminoglycan synthesis, affected glycosaminoglycan sulphation, and also reduced beta-N-acetyl-D-glucosaminidase, beta-N-acetyl-D-galactosaminidase and beta-glucuronidase activities.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antibodies, Monoclonal / immunology
-
Cells, Cultured
-
Chick Embryo
-
Concanavalin A / pharmacology*
-
Cytoskeleton / drug effects
-
Cytoskeleton / ultrastructure*
-
DNA / biosynthesis
-
Fibroblasts / drug effects
-
Fibroblasts / metabolism
-
Fibroblasts / ultrastructure
-
Fluorescent Antibody Technique
-
Glycosaminoglycans / metabolism*
-
Glycoside Hydrolases / metabolism*
-
Leucine / metabolism
-
Microscopy, Electron, Scanning
-
Protein Biosynthesis
-
Proteoglycans / metabolism
-
Sulfates / metabolism
-
Thymidine / metabolism
Substances
-
Antibodies, Monoclonal
-
Glycosaminoglycans
-
Proteoglycans
-
Sulfates
-
Concanavalin A
-
DNA
-
Glycoside Hydrolases
-
Leucine
-
Thymidine