We investigated the combined effects of hyaluronan and fibronectin on corneal epithelial wound closure in vivo. When fibronectin eye drops (1 mg/ml) were administered for the first 4 hours after chemical cautery and hyaluronan eye drops (1 mg/ml) were administered for following 32 hours, the mean healing rate was significantly higher than in control eyes treated with phosphate buffered saline instead of fibronectin or with initial fibronectin alone. These results demonstrated that hyaluronan and fibronectin have a synergistic effect, with fibronectin pretreatment augmenting hyaluronan-stimulated corneal epithelial wound closure.