Genotyping ocular strains of Chlamydia trachomatis by single-tube nested PCR

S Pecharatana; M A Pickett; P J Watt; M E Ward

doi:10.1101/gr.3.3.200

Genotyping ocular strains of Chlamydia trachomatis by single-tube nested PCR

PCR Methods Appl. 1993 Dec;3(3):200-4. doi: 10.1101/gr.3.3.200.

Authors

S Pecharatana¹, M A Pickett, P J Watt, M E Ward

Affiliation

¹ Department of Molecular Microbiology, University Medical School, Southampton General Hospital, UK.

PMID: 8118403
DOI: 10.1101/gr.3.3.200

Abstract

A single-tube nested PCR able to discriminate between ocular infection with Chlamydia trachomatis of serovar A, B, or C is described. The method uses genotype-specific primers labeled with different fluorochromes, "drop-in/drop-out" PCR amplification, and product analysis on an Applied Biosystems 373A DNA Sequencer using GENESCAN 672 software. The system readily detected mixed infection with serotypes A and B within the same eye. This strategy provides a rapid genotyping method applicable to a wide variety of epidemiological studies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Chlamydia Infections / epidemiology
Chlamydia Infections / microbiology*
Chlamydia trachomatis / genetics*
DNA Primers
Electrophoresis, Polyacrylamide Gel
Eye Infections, Bacterial / epidemiology
Eye Infections, Bacterial / microbiology*
Fluorescent Dyes
Gambia / epidemiology
Genotype
Humans
Molecular Sequence Data
Polymerase Chain Reaction / methods*

Substances

DNA Primers
Fluorescent Dyes