Abstract
We have been using a differential cDNA cloning approach to isolate human retina-specific and retina-enriched genes [1]. A 1,314 bp cDNA was isolated by this approach, representing a highly retina-specific message encoding a 388 amino acid protein showing 58%, 50%, and 49% homology to bovine beta-arrestin, and bovine and human retinal arrestin (S-antigen), respectively. Chromosomal mapping localized this new arrestin gene to the proximal long arm of the X chromosome, hence it was named X-arrestin. In situ hybridization demonstrated its expression in the inner and outer segments and the inner plexiform regions of the retina.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens / biosynthesis
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Antigens / genetics*
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Arrestin
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Arrestins*
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Base Sequence
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Blotting, Northern
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Cattle
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Chromosome Mapping
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Cloning, Molecular
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DNA, Complementary
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Eye Proteins / biosynthesis
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Eye Proteins / genetics*
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Gene Library
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Humans
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In Situ Hybridization
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Membrane Proteins / genetics*
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Molecular Sequence Data
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Pigment Epithelium of Eye / metabolism
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RNA, Messenger / isolation & purification
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RNA, Messenger / metabolism
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Retina / metabolism*
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Sequence Homology, Amino Acid
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Thyroid Gland / metabolism
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X Chromosome*
Substances
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Antigens
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Arrestin
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Arrestins
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DNA, Complementary
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Eye Proteins
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Membrane Proteins
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RNA, Messenger
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arrestin3