A new casting technique for studying the three-dimensional structure of the human retinal vascular system on post mortem tissue was developed. The technique was designed to provide an overview presentation of the retinal vasculature such that localized areas with vascular pathology could be identified for further analysis of three-dimensional structure. The paper presents qualitative characteristics of three-dimensional structure in the normal human retinal vascular bed, to provide a basis for future studies on vascular impairment in retinal disease.