Functional group gradient surfaces where the surface density of grafted functional groups changes gradually along the sample length were prepared on low density polyethylene (PE) sheets by corona discharge treatment with gradually increasing power and graft copalymerization of acrylic acid (AA), sodium p-styrene sulphonate (NaSS), and N,N-dimethyl aminopropyl acrylamide (DMAPAA). AA and NaSS are negatively chargeable and DMAPAA is positively chargeable in phosphate buffered saline or cell culture medium at pH 7.3-7.4. The functional group gradient surfaces were characterized by the measurement of water contact angle, Fourier transform infrared spectroscopy in the attenuated total reflectance mode, and electron spectroscopy for chemical analysis. All these measurements indicated that the functional groups were grafted on the PE surfaces with gradual increase of their density. The interaction of Chinese hamster ovary cells with the functional group gradient surfaces along the sample length was investigated. The cells that had adhered and grown on the surfaces were counted and observed by scanning electron microscopy. It was observed that a greater quantity of the cells had adhered and grown onto the positions with moderate density of the functional groups. This may be related to the hydrophilicity of the surface. The DMAPAA-grafted surface showed a large amount of cell attachment probably owing to the positive charge character, while the AA-grafted surface, which is negatively charged, showed poor cell attachment, as expected. The NaSS-grafted surface which is also negatively charged showed a large amount of cell attachment. This may be closely associated with the existence of an aromatic ring close to the ionizable group in NaSS. It seems that surface functional groups and their charge character as well as wettability play important roles for cell adhesion, spreading, and growth.